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mouse anti human tlr4 fitc  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology mouse anti human tlr4 fitc
    Mouse Anti Human Tlr4 Fitc, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 833 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mouse anti human tlr4 fitc/product/Santa Cruz Biotechnology
    Average 96 stars, based on 833 article reviews
    mouse anti human tlr4 fitc - by Bioz Stars, 2026-03
    96/100 stars

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    Santa Cruz Biotechnology fitc-conjugated mouse anti-human tlr4 antibody
    Whole blood from healthy volunteers (HV, n = 14) or major trauma patients (TP, n = 29) was analyzed over a 10-day time course after admission (emergency department, ED–10) by flow cytometry. Monocytes were detected using anti-human CD14 in the corresponding sideward and forward scatter. Unstimulated (black symbols) and stimulated (clear symbols) measurements were made. For stimulation (stim), whole blood was incubated with leukocyte activation cocktail for 5 h with subsequent analyzing procedure as in unstimulated samples. Data are shown as mean ± SEM. (a) TLR2 expression in CD14 + monocytes, (b) <t>TLR4</t> expression in CD14 + monocytes, (c) TLR9 expression in CD14 + monocytes, (d) TLR2 expression in CD14 + HLA-DR − monocytes, (e) TLR4 expression in CD14 + HLA-DR − monocytes, and (f) TLR9 expression in CD14 + HLA-DR − monocytes. ∗ p < 0.05 versus unstimulated HV; ⌀ p < 0.05 versus stimulated HV; æ p < 0.05 TP versus corresponding TP stim; # p < 0.05 HV ctrl versus all; & p < 0.05 versus all; ∞ p < 0.05 TP stim versus corresponding unstimulated TP and HV stim.
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    Whole blood from healthy volunteers (HV, n = 14) or major trauma patients (TP, n = 29) was analyzed over a 10-day time course after admission (emergency department, ED–10) by flow cytometry. Monocytes were detected using anti-human CD14 in the corresponding sideward and forward scatter. Unstimulated (black symbols) and stimulated (clear symbols) measurements were made. For stimulation (stim), whole blood was incubated with leukocyte activation cocktail for 5 h with subsequent analyzing procedure as in unstimulated samples. Data are shown as mean ± SEM. (a) TLR2 expression in CD14 + monocytes, (b) TLR4 expression in CD14 + monocytes, (c) TLR9 expression in CD14 + monocytes, (d) TLR2 expression in CD14 + HLA-DR − monocytes, (e) TLR4 expression in CD14 + HLA-DR − monocytes, and (f) TLR9 expression in CD14 + HLA-DR − monocytes. ∗ p < 0.05 versus unstimulated HV; ⌀ p < 0.05 versus stimulated HV; æ p < 0.05 TP versus corresponding TP stim; # p < 0.05 HV ctrl versus all; & p < 0.05 versus all; ∞ p < 0.05 TP stim versus corresponding unstimulated TP and HV stim.

    Journal: Mediators of Inflammation

    Article Title: Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients

    doi: 10.1155/2017/2608349

    Figure Lengend Snippet: Whole blood from healthy volunteers (HV, n = 14) or major trauma patients (TP, n = 29) was analyzed over a 10-day time course after admission (emergency department, ED–10) by flow cytometry. Monocytes were detected using anti-human CD14 in the corresponding sideward and forward scatter. Unstimulated (black symbols) and stimulated (clear symbols) measurements were made. For stimulation (stim), whole blood was incubated with leukocyte activation cocktail for 5 h with subsequent analyzing procedure as in unstimulated samples. Data are shown as mean ± SEM. (a) TLR2 expression in CD14 + monocytes, (b) TLR4 expression in CD14 + monocytes, (c) TLR9 expression in CD14 + monocytes, (d) TLR2 expression in CD14 + HLA-DR − monocytes, (e) TLR4 expression in CD14 + HLA-DR − monocytes, and (f) TLR9 expression in CD14 + HLA-DR − monocytes. ∗ p < 0.05 versus unstimulated HV; ⌀ p < 0.05 versus stimulated HV; æ p < 0.05 TP versus corresponding TP stim; # p < 0.05 HV ctrl versus all; & p < 0.05 versus all; ∞ p < 0.05 TP stim versus corresponding unstimulated TP and HV stim.

    Article Snippet: Blood samples (100 μ l) were transferred into polystyrene FACS tubes (BD Pharmingen) and incubated with mouse anti-human CD14 V500 (Clone M5E2, BD Bioscience, San Jose, CA), mouse anti-human TLR2 PE-Cy7 (Clone T2.5, eBioscience, San Diego, CA), mouse anti-human TLR4 FITC (Clone HTA125, IMGENEX, San Diego, CA), mouse anti-human TLR9 Alexa Flour® 647 (Clone 26C593.2, IMGENEX, San Diego, CA), and mouse anti-human HLA-DR PerCP-Cy5.5 conjugated (Clone L243, BD Bioscience, San Jose, CA) antibodies.

    Techniques: Flow Cytometry, Incubation, Activation Assay, Expressing

    Whole blood from healthy volunteers (HV, n = 14) or major trauma patients (TP, n = 29) was analyzed by flow cytometry over a 10-day time course after admission (emergency department, ED–10). Monocytes were detected using anti-human CD14 in the corresponding sideward and forward scatter. Unstimulated (black symbols) and stimulated (clear symbols) measurements were made. For stimulation (stim), whole blood was incubated with leukocyte activation cocktail for 5 h with subsequent analyzing procedure as in unstimulated samples. Data are shown as mean ± SEM. (a) TLR2/HLA-DR coexpression on CD14 + monocytes, (b) TLR4/HLA-DR coexpression on CD14 + monocytes, and (c) TLR9/HLA-DR coexpression on CD14 + monocytes. ∗ p < 0.05 versus unstimulated HV; æ p < 0.05 TP versus corresponding TP stim; & p < 0.05 versus all; ∞ p < 0.05 TP stim versus corresponding unstimulated TP and HV stim.

    Journal: Mediators of Inflammation

    Article Title: Impaired Surface Expression of HLA-DR, TLR2, TLR4, and TLR9 in Ex Vivo-In Vitro Stimulated Monocytes from Severely Injured Trauma Patients

    doi: 10.1155/2017/2608349

    Figure Lengend Snippet: Whole blood from healthy volunteers (HV, n = 14) or major trauma patients (TP, n = 29) was analyzed by flow cytometry over a 10-day time course after admission (emergency department, ED–10). Monocytes were detected using anti-human CD14 in the corresponding sideward and forward scatter. Unstimulated (black symbols) and stimulated (clear symbols) measurements were made. For stimulation (stim), whole blood was incubated with leukocyte activation cocktail for 5 h with subsequent analyzing procedure as in unstimulated samples. Data are shown as mean ± SEM. (a) TLR2/HLA-DR coexpression on CD14 + monocytes, (b) TLR4/HLA-DR coexpression on CD14 + monocytes, and (c) TLR9/HLA-DR coexpression on CD14 + monocytes. ∗ p < 0.05 versus unstimulated HV; æ p < 0.05 TP versus corresponding TP stim; & p < 0.05 versus all; ∞ p < 0.05 TP stim versus corresponding unstimulated TP and HV stim.

    Article Snippet: Blood samples (100 μ l) were transferred into polystyrene FACS tubes (BD Pharmingen) and incubated with mouse anti-human CD14 V500 (Clone M5E2, BD Bioscience, San Jose, CA), mouse anti-human TLR2 PE-Cy7 (Clone T2.5, eBioscience, San Diego, CA), mouse anti-human TLR4 FITC (Clone HTA125, IMGENEX, San Diego, CA), mouse anti-human TLR9 Alexa Flour® 647 (Clone 26C593.2, IMGENEX, San Diego, CA), and mouse anti-human HLA-DR PerCP-Cy5.5 conjugated (Clone L243, BD Bioscience, San Jose, CA) antibodies.

    Techniques: Flow Cytometry, Incubation, Activation Assay